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Resume of KNI, PhD
Consultant

EXPERTISE AND SERVICES

Immunology Expert Consultant, Biotechnologist
Expertise in biotechnology, microbiology, cytology, virology, and immunology, with special interests in the biology of cancer, and molecular genetics.

Specialization includes improved inoculation of cultured plant cells, freezing of plant cells and protoplasts, plasma biomarker for precancerous lesions of the bowel, milk and u.s. cancer mortality, citrus and prevention of colon cancer, use of cell cultures to identify human precancerous condition, and herpes simplex virus.

Has conducted numerous studies in areas including electron microscopy virus, vaccinium corymbosum, phytopathology, immunological methods for the agglutination of protoplasts from cell suspension cultures of different genera, and fusion of erythrocytes by treatment with proteolytic enzymes and polyethylene glycol.

Extensive knowledge regarding electron microscopic study of plant-animal cell fusion, molecular marine biology and biotechnology, studies of viruses and bacteria in marine water adjacent to sewage outfalls, attenuated virus vaccine for the prevention of channel catfish virus disease, ascorbate, cyclic nucleotides, and a cell fusion vaccine for the treatment of chronic lymphocytic leukemia

PRINCIPAL INDUSTRIES SERVED
Bio-Technology, Medical, Pharmaceuticals

EDUCATION
Post Doc., National Research Council
Ph.D., Michigan State University
M.S., Michigan State University
B.S., Aquinas College

PROFESSIONAL EXPERIENCE
Consultant, Kevin Kennedy & Associates, Inc.

Providing biotechnology, microbiology, cytology, virology, and immunology consulting and related expertise to a wide variety of clients.

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Professor of Biological Sciences, major medical research university

Provided instruction in Cell Biology, Molecular Genetics and Cytology, Virology-Immunology, Virology, Biology of Cancer, Immunology, Animal and Plant Cell Culture, Microbiology.

ADDITIONAL EXPERIENCE

RESEARCH ACCOMPLISHMENTS
Member of a team that produced the first plant virus-plant tissue culture system
Established the etiology of mycoplasma and virus diseases of blueberries
First to fuse plant with animal cells
Produced the first herpes virus vaccine for fish, now studied as a model system for human herpes
Found a plasma bio-marker for human intestinal polyps
Produced the first monoclonal antibody-based fish identification kit for billfish conservation
Produced the first continuous shark cell line
Co-founder: Leukemia and Lymphoma Research Foundation

GRANTS AND AWARDS
Isolation, enumeration and characterization of viruses in marine waters
Development of an infectivity assay system for plant viruses: hypersensitive protoplasts isolated by enzymatic treatment of plant tissue culture cells
Studies on pollution in marine waters adjacent to sewage outfalls
The fusion of cultured cell protoplasts by membrane specific antibody molecules couples to membrane lytic agents
The inoculation of aquatic weeds with plant viruses
Studies on plant cell fusion seed grant
Production of antibodies to plant protoplasts
Fusion of plant and animal cells
Studies on the immune response of chickens of rous sarcoma virus tumors
Comparison of fusion abilities of normal and malignant cultured cells
Studies on the vaccination of rats with multinucleate tumor cells
Studies on the assay of immune blocking factor by lymphocyte blastogenesis
Biological control of the walking catfish
Biological control of the problematic walking catfish;
Vaccination of channel catfish
Determination of cyclic nucleotide levels in normal and society
Development of a blood test or polyps
Isolation of biomarker proteins from makaira nigricans, tetrapturus albidus, and istiophorus albicans using polyacrylamide gel isoelectric focusing
International commission for the conservation of atlantic tunas determination of the sensitivity and specificity of polyclonal antisera produced against billfish white muscle proteins
Why sharks do not get cancer
Shark cell cultures as a source of anti-angiogenin and a physiological model billfish
An antibody based method for the determination of seafood freshness
Production of an antibody-based kit for the identification of billfish carcasses
Evaluation of the sensitivity and specificity of monoclonal antibodies produced against fish albumins
Fellowship from applied biomaterials technologies
Foundation donation for cancer research n the immunotherapy of melanoma
Effect of echinacea on the regression of breast cancer in a mouse model following chemotherapy treatment
Studies on the isolation of heat shock peptide complexes as immunotherapeutic vaccines
Requisites for the generation of cytotoxic t lymphocytes in patients with chronic lymphocytic leukemia
A comparison of methods used in somatic cell fusion
Transmission and scanning electron microscopy studies on the fusion of avian erythrocytes
The effect of macrophage on rous sarcoma virus transformed wistar rat fibroblasts
Cryopreservation of plant protoplasts
Studies on polyethylene glycol mediated aggregation and fusion of avian erythrocytes
Studies on channel catfish virus passage in cell cultures of the walking catfish
In vitro characterization of channel catfish virus vaccine
Interferon induction by channel catfish virus infection in fish cell culture
Effectiveness of anti-herpetic
Compounds on pacheco's disease virus of psittacines
Evaluation of factors influencing sporulation in the maxine deuteromycete trichocladium achrasporum
Identification of influenza type a virus using a fluorogenic neuraminidase assay
Comparison of shark and hamster cell lines response to carcinogenic chemicals
Establishment and study of two elasmobranch cell lines
Evaluation of the sensitivity and specificity of monoclonal antibodies produced against sailfish albumin
Rapid immunologic identification of panniker,
Monoclonal antibodies produced against blue marlin species
Immunoaffinity purification of albumin from fish serum
Evaluation of monoclonal technology for the species specific identification of centropomus undecimalis
Cytokinetic effects of platinum polyamines on three different cell lines using flow cytometry
Istiophorus albicans-optimization of an immunoassay- based identification kit

TECHNOLOGICAL ACCOMPLISHMENTS
Electron Microscopy
Radioisotope labeling of Nucleic Acids
Polyacrylamide gel electrophoresis
Isoelectric Focusing
Cell Culture - animal, plant, fish, avian and shark
Hybridoma selection and production
Monoclonal antibody production and purification
High pressure liquid chromatography of Proteins
Affinity Chromatography
Enzyme Linked Immunoassay
Virus Culture, Purification

PUBLICATIONS
Numerous publications pertaining to biotechnology, microbiology, cytology, virology, and immunology.

Please note that this is an abbreviated CV. A fully detailed unabridged CV is available under special circumstances.

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Kevin Kennedy & Associates, Inc.
Rapid Response Engineering® Solutions
3905 Vincennes Road, Suite 320
Indianapolis, Indiana 46268
(317) 536-7000 voice
(317) 536-7220 fax

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