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Resume of TUU, Ph.D.
Consultant

EXPERTISE AND SERVICES

Biochemist, Biotechnologist, Macromolecular Crystallography Specialist, Consultant, Forensic Analysis Investigation Expert Witness Testimony, Consulting, Services
Expertise in molecular biology and techniques related to site-directed mutagenesis, plasmid construction, and cloning recombinant DNA, growth, maintenance, and manipulation of microorganism (phage, and bacteria), DNA sequencing.

Additional areas of expertise include overexpression of recombinant enzymes, protein chemistry/enzymology, protein purification by affinity chromatography, gel filtration, ion exchange chromatography using FPLC/HPLC and classical methods, enzymological assay techniques for determining activity.

Highly knowldegeable in areas including thermal stability, protein-DNA interactions via cross linking, and polymerase catalyzed DNA synthesis, protein crystallography, crystallization of protein and protein-DNA complexes in the presence of inhibitors, cryo-techniques for crystal cooling and synchrotron data collection.

Studies relative to polymerase and exonuclease function of Escherichia coli DNA Polymerase I, anti-AIDS drug Tenofovir, nonnucleoside inhibitor binding, HIV-1 reverse transcriptase with pre- and post-translocation AZTMP-terminated DNA

PRINCIPAL INDUSTRIES SERVED
Biotechnology

EDUCATION
Post-doctoral Fellow in Macromolecular Crystallography, The Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ.
Ph.D., Biochemistry, University of Medicine and Dentistry of New Jersey, Graduate School of Biomedical Sciences, Newark, New Jersey
B.S., Biotechnology, Magna Cum Laude, William Paterson College of New Jersey, Wayne, New Jersey

PROFESSIONAL EXPERIENCE
Consultant, Kevin Kennedy & Associates, Inc.

Providing biochemistry, biotechnology, macromolecular crystallography expertise, consulting, and related expertise to a wide variety of clients.

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Biochemist, Biotechnologist, Consultant

Molecular Biology: Proficiency with molecular biological techniques related to site-directed mutagenesis including: Plasmid construction, and cloning recombinant DNA. Growth, maintenance, and manipulation of microorganism (phage, and bacteria). DNA sequencing, overexpression of recombinant enzymes.
Protein Chemistry/Enzymology: Protein purification by affinity chromatography Gel filtration, Ion exchange chromatography using FPLC/HPLC and classical methods, Experienced in enzymological assay techniques for determining activity, thermal stability, protein-DNA interactions via cross linking, and steady state kinetic analysis of polymerase catalyzed DNA synthesis.
Protein Crystallography: Experienced in the crystallization of protein and protein-DNA complexes in the presence of inhibitors. Fundamental knowledge of cryo-techniques for crystal cooling and synchrotron data collection.
Computer Skills: Basic knowledge of macromolecular databases and modeling software including: O, SYBYL, and MOLMOL. Proficiency with UNIX, CNS, HKL.

HONORS AND AWARDS
C. Kent Warner Award for Academic Excellence, William Paterson College, NJ
National Research Service Award Research Fellowship, National Institutes of Health, National Institute of Allergy and Infectious Diseases

PUBLICATIONS
Numerous publications on topics related to residues in polymerase and exonuclease function of Escherichia coli DNA Polymerase I, the anti-AIDS drug Tenofovir, nonnucleoside inhibitor binding, HIV-1 reverse transcriptase with pre- and post-translocation AZTMP-terminated DNA, and more.

Please note that this is an abbreviated CV. A fully detailed unabridged CV is available under special circumstances.

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Kevin Kennedy & Associates, Inc.
Rapid Response Engineering® Solutions
3905 Vincennes Road, Suite 320
Indianapolis, Indiana 46268
(317) 536-7000 voice
(317) 536-7220 fax

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