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ENGINEERING AND SCIENTIFIC CONSULTING SERVICES
Resume of DYH, Ph.D.
Consultant
EXPERTISE AND SERVICES
Biochemist, Molecular Biologist, Research Scientist, Manager, Consultant, Specialist, Forensic Analysis Investigation Expert Witness Testimony, Consulting ServicesExpertise in protein purification using FPLC, HPLC, cloning, PCR, DNA purification, DNA sequencing, gel shifts, western, northern, southern blotting, primer extension assays, cell culture, DNA and RNA transfection, membrane-based lateral flow diagnostic assays, membrane technology and applications, high throughput device design and optimization, life science applications, and environmental analysis.
Specialization includes the molecular biology of human immunodeficiency virus replication, development/support of high throughput devices and applications for the genomics industry, molecular biology kits for DNA purification, product validation, quality documentation, and preparation of technical literature, technical support, sales, marketing, and technical services for genomics applications, quality control, product development, marketing communications, packaging, supplier quality, and purchasing.
Extensive knowledge regarding generation, purification, and biophysical characterization of mutant proteins, isolating HIV-1 pre-integration complexes, molecular modeling programs, protein structures, human protein (BAF) involved in HIV integration, mutational analysis of HIV-1 reverse transcriptase, amino acid residues that influence dNTP substrate binding and/or drug resistance, analytical instruments and equipment, water and soil analysis, and pesticides.
Has conducted numerous studies in areas including Glutamine 151 of Human Immunodeficiency Virus Type 1 reverse transcriptase and RNA directed DNA synthesis, The p51 subunit of Human Immunodeficiency Type 1 reverse transcriptase, polymerase activity loss due to mutation in the HIV-1 reverse transcriptase, amino acid residues and HIV-1 reverse transcriptase, Glutamine 151 of HIV Type-1 reverse transcriptase and substrate selection, the reconstitution of HIV type 1 integration in vitro, hi speed plasmid isolation, insertion of six amino acids into the HIV-1 reverse transcriptase and heterodimer activity, and purification of BAC DNA templates
PRINCIPAL INDUSTRIES SERVED
Biotechnology, Pharmaceutical
EDUCATION
Ph.D., Biochemistry & Molecular Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ
B.A., Liberal Studies/Chemistry, California State University, Fresno, CA
PROFESSIONAL EXPERIENCE
Consultant, Kevin Kennedy & Associates, Inc.
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Providing biochemistry consulting, molecular biology, research science, management, consulting, and related expertise to a wide variety of clients.
Speak to this experienced, world-class expert now.
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Molecular Biologist, Management Consultant
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Was responsible for managing consultants and projects. Was responsible for marketing, advertising, and partnering activities. Was responsible for delivering customer training courses. Writing and editing services for clients.
Senior Scientist, Genomics and Molecular Biology, a Bioscience Company providing technologies for new therapeutic drug development
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Served as Group leader for the development of molecular biology kits for DNA purification. Developed and launched a new product line of molecular biology kits, resulting in $2M of new sales in the first year. Managed product validation, quality documentation, and preparation of technical literature. Provided critical technical support for major accounts, which contributed to the closing of over $1M in new business. New customers included several of the largest public and private genome research centers. Served as in house consultant to sales, marketing, and technical services for entire range of genomics applications. Completed a technical seminar tour through Asia and Australia introducing novel platforms for DNA purification. Provided ongoing product training to Sales and Technical Support Groups in North America, Asia, Europe, and Australia. Negotiated with vendors for sourced reagents and collaboratively drafted quality plans for production and Q.C. testing. Worked closely with other groups/departments in coordinating product development such as marketing, marketing communications, packaging, supplier quality, purchasing, and sales.
Research Fellow, Dana-Farber Cancer Institute (Department of Cancer Immunology & AIDS) & Harvard Medical School (Department of Pathology), Boston, MA
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Designed experimental strategies toward elucidation of HIV-1 integrationƒn (generation, purification, and biophysical characterization of mutant proteins). Developed novel means of isolating HIV-1 pre-integration complexes (PICs) through the insertion of epitope tags using overlapping PCR. Used molecular modeling programs to analyze protein structures, presented experimental data at local and international scientific meetings, and prepared manuscripts for publication in peer reviewed journals. Delivered oral presentation of research on a novel human protein (BAF) involved in HIV integration at the international meeting on retroviruses at Cold Spring Harbor National Laboratories. Supported by National Research Service Award (NRSA) Grant from the National Institutes of Health.
Graduate Student, University of Medicine and Dentristry of New Jersey, Graduate School of Biomedical Sciences, Newark, NJ
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Thesis work consisted of mutational analysis of HIV-1 reverse transcriptase and specifically identification of amino acid residues that influence dNTP substrate binding and or/drug resistance (publications below).
Pesticide Chemist, Anametrix Laboratories, San Jose, CA
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Maintained and operated analytical instruments and equipment. Acquired and analyzed data usingƒnspecialized software. Managed large projects for clients, implementing the highest level of quality control in environmental analysis. Designed a new system for tracking projects and streamlining production. Trained and managed employees.
Pesticide Chemist, The Twining Laboratories, Fresno, CA
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Prepared water and soil samples for analysis by chromatographic methods. Maintained and operated analytical instruments in the pesticide division of an environmental chemistry lab. Trained and supervised employees.
PUBLICATIONS
Numerous publications on topics related to Glutamine 151 of Human Immunodeficiency Virus Type 1 reverse transcriptase and RNA directed DNA synthesis, The p51 subunit of Human Immunodeficiency Type 1 reverse transcriptase, polymerase activity loss due to mutation in the HIV-1 reverse transcriptase, amino acid residues and HIV-1 reverse transcriptase, Glutamine 151 of HIV Type-1 reverse transcriptase and substrate selection, the reconstitution of HIV type 1 integration in vitro, hi speed plasmid isolation, insertion of six amino acids into the HIV-1 reverse transcriptase and heterodimer activity, purification of BAC DNA templates, and more
Presented experimental data at local and international scientific meetings. Delivered oral presentation of research on a novel human protein (BAF) involved in HIV integration at the international meeting on retroviruses
Please note that this is an abbreviated CV. A fully detailed unabridged CV is available under special circumstances.
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Kevin Kennedy & Associates, Inc.
Rapid Response Engineering® Solutions
3905 Vincennes Road, Suite 320
Indianapolis, Indiana 46268
(317) 536-7000 voice
(317) 536-7220 fax