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Resume of CAW, Ph.D.
Consultant

EXPERTISE AND SERVICES

Chemist, Biochemist, Biomedical Research Scientist, Consultant, Specialist, Forensic Analysis Investigation Expert Witness Testimony, Consulting Services
Expertise in pharmacology, biophysics, molecular biology, pathology, laboratory medicine, mouse brain dissection, synaptosome preparation, neuronal cell culture, mouse breeding and flow cytometry, cell culture and transfection, cell fractionation, northern and western blotting, immunoprecipitation, recombinant DNA cloning, RT-PCR, fluorescent microscopy and sequence alignment analysis, bacterial protein expression.

This KKAI Associate offers expertise specific to mRNA isolation, in vitro AUrich RNA selection by affinity chromatography, cDNA synthesis, cDNA library screening, BLAST homology searches, particle mediated gene transfer, PBMC isolation, in vitro transcription and RNA purification, northern hybridization analysis, SDS-PAGE and ECL western blot analysis, ELISA, PCR, RNA gel mobility shift assays, cell culture, protein purification and tat-mediated protein transfection, protein kinase assays, sequence analysis, in vitro phosphorylation, protein digestion and peptide isolation, amino acid hydrolysis.

Additional experience and specialization includes identification of phosphoamino acids by thin layer chromatography, oocyte isolation and in vivo radiolabeling of proteins, affinity purification of antibodies, immunoprecipitation, expression and purification of recombinant protein in E.coli, SDS-PAGE, autoradiography and densitometry, protein and nucleic acid purification, isolation of eukaryotic ribosomes, electrophoresis, germinal vesicle isolation, preparation of whole cell and nuclear transcription extracts, DNase I and copper phenanthroline footprinting, quantitative footprinting, in vitro transcription assays in extract, gel mobility shift assays, western blot analysis, molecular cloning, hybridization analysis.

Highly experienced in areas including DNA and RNA sequencing, radioisotope usage and small animal dissection, influenza virus receptors, slow viruses, Scrapie, Mad Cow and Kuru, UV-light and apoptotic signal transduction, nervous system disorders, Alzheimer's disease, mouse hippocampus, RhoB mRNA and UV light, phosphorylation of xenopus transcription factor IIIA, extracellular regulated kinase controls, upregulation of nucleolin mRNA and protein, inhibition of RNA polymerase III transcription, amyloid precursor protein (APP) production, neurodegenerative disorders, RNA binding protein HuR, ribosomes and the transcription of RNA Polymerase III genes

PRINCIPAL INDUSTRIES SERVED
Biotechnology, Research, Medical, Pharmaceutical

EDUCATION
Ph. D., Biochemistry, University of Notre Dame, Notre Dame, IN
B.S., Chemistry, Clarke College, Dubuque, IA

PROFESSIONAL EXPERIENCE
Consultant, Kevin Kennedy & Associates, Inc

Providing chemistry consulting, biochemistry consulting, biomedical research, and related expertise to a wide variety of clients.

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Chemist, Biochemist, a major research university

Researched the Identification of Novel FMRP mRNA Targets in Neurodegenerative Disorders. Techniques Employed: mouse brain dissection, synaptosome preparation, neuronal cell culture, mouse breeding and flow cytometry.

Researched the Post-Transcriptional Regulation of RhoB mRNA by the RNA Binding Protein HuR. Techniques Employed: cell culture and transfection, cell fractionation, northern and western blotting, immunoprecipitation, recombinant DNA cloning, RT-PCR, fluorescent microscopy and sequence alignment analysis.

Researched the Isolation of Novel Early Response Genes from the Central Nervous System in a Mouse Epilepsy Model. Techniques Employed: bacterial protein expression, mRNA isolation, in vitro AUrich RNA selection by affinity chromatography, cDNA synthesis, cDNA library screening, BLAST homology searches.

Additional Training
Mentor to graduate and undergraduate students, training in mouse handling and preparation of R01 grant applications.

Postdoctoral Research Fellow, Department of Pathology & Laboratory Medicine and Institute on Aging, University of Wisconsin-Madison

Researched The Molecular Biology of Alzheimer’s Disease. Techniques Employed: particle mediated gene transfer, PBMC isolation, in vitro transcription and RNA purification, northern hybridization analysis, SDS-PAGE and ECL western blot analysis, ELISA, PCR, RNA gel mobility shift assays, cell culture, protein purification and tat-mediated protein transfection, protein kinase assays, sequence analysis

Postdoctoral Research Associate, Department of Chemistry and Biochemistry, University of Notre Dame

Researched Continuation of Dissertation Projects described below

Graduate Student, Biophysics, and Molecular Biology Training Program, Department of Chemistry and Biochemistry and Biochemistry, University of Notre Dame

Self-Directed Dissertation Project: The Phosphorylation of Xenopus Transcription Factor IIIA by an Oocyte Casein Kinase. Techniques Employed: in vitro phosphorylation, protein digestion and peptide isolation, amino acid hydrolysis, identification of phosphoamino acids by thin layer chromatography, oocyte isolation and in vivo radiolabeling of proteins, affinity purification of antibodies, immunoprecipitation, expression and purification of recombinant protein in E.coli, SDS-PAGE, autoradiography and densitometry

Primary Dissertation Project: The Effect of Ribosomes on the Transcription of RNA Polymerase III Genes. Techniques Employed: protein and nucleic acid purification, isolation of eukaryotic ribosomes, electrophoresis, germinal vesicle isolation, preparation of whole cell and nuclear transcription extracts, DNase I and copper phenanthroline footprinting, quantitative footprinting, in vitro transcription assays in extract, gel mobility shift assays, western blot analysis, molecular cloning, hybridization analysis, DNA and RNA sequencing

Additional Training
Certification in radioisotope usage and small animal dissection.

HONORS AND AWARDS
New Investigator Award for Excellence in Biomedical Research, University of Wisconsin-Madison
Postdoctoral Trainee Appointment, Institute on Aging on the Biology of Aging and Age-Related Diseases Training Grant (NIH)
Rohm & Haas Outstanding Graduate Student Award (Honorable Mention), University of Notre Dame
Reilly Fellowship
Zahm Fellowship
Clarke College: Sr. Marguerite Neumann Chemistry Award
Kappa Gamma Pi National Catholic Graduate Honor Society
Presidential Scholarship
Alumni Scholarship
State of Iowa Scholarship
Iowa Math & Science Tuition Grant
Wm. C. Brown/KLYV/WDBQ Scholarship

PUBLICATIONS
Numerous publications on topics related to mouse hippocampus, RhoB mRNA and UV light, phosphorylation of xenopus transcription factor IIIA, extracellular-regulated kinase controls, upregulation of nucleolin mRNA and protein, inhibition of RNA polymerase III transcription, amyloid precursor protein (APP) production, and more.

Numerous presentations on topics related to UV-light and apoptotic signal transduction, nervous system disorders, Alzheimer's disease, and more.

Please note that this is an abbreviated CV. A fully detailed unabridged CV is available under special circumstances.

Read articles by this associate:

FMRP Mediates mGluR5-Dependent Translation of Amyloid Precursor Protein

HuR mRNA ligands expressed after seizure

RhoB mRNA is stabilized by HuR after UV light

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Kevin Kennedy & Associates, Inc.
Rapid Response Engineering® Solutions
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Indianapolis, Indiana 46268
(317) 536-7000 voice
(317) 536-7220 fax

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