Experts
EXPERTLY ORCHESTRATED GENIUS
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Resume of CAW, Ph.D.
Consultant
PRINCIPAL INDUSTRIES SERVED
- Biotechnology
- Research
- Medical
- Pharmaceutical
EDUCATIONThis expert has a Ph.D. in Biochemistry from the University of Notre Dame in Notre Dame, IN.
This expert has a B.S. in Chemistry from Clarke College in Dubuque, IA. EXPERTISE AND SERVICESChemist, Biochemist, Biomedical Research Expert Consultant ResumeThis expert specializes in pharmacology, biophysics, molecular biology, and pathology. Further expertise includes laboratory medicine, mouse brain dissection, synaptosome preparation, and neuronal cell culture. Other related fields of expertise include mouse breeding and flow cytometry, cell culture and transfection, cell fractionation, and northern and western blotting. This expert's specialties also include immunoprecipitation, recombinant DNA cloning, RT-PCR, fluorescent microscopy and sequence alignment analysis and bacterial protein expression.
This expert offers expertise specific to mRNA isolation in vitro AUrich RNA selection by affinity chromatography, cDNA synthesis, cDNA library screening, and BLAST homology searches. More areas include particle mediated gene transfer, PBMC isolation, in vitro transcription and RNA purification, and northern hybridization analysis. More areas of expertise are SDS-PAGE and ECL western blot analysis, ELISA, PCR, RNA gel mobility shift assays, cell culture, protein purification and tat-mediated protein transfection. This expert also has experience with protein kinase assays, sequence analysis, in vitro phosphorylation, protein digestion and peptide isolation, and amino acid hydrolysis.
Additional experience and specialization includes identification of phosphoamino acids by thin layer chromatography, oocyte isolation and in vivo radiolabeling of proteins, affinity purification of antibodies, immunoprecipitation, expression and purification of recombinant protein in E.coli, SDS-PAGE, autoradiography and densitometry, protein and nucleic acid purification, isolation of eukaryotic ribosomes, electrophoresis, germinal vesicle isolation, preparation of whole cell and nuclear transcription extracts, DNase I and copper phenanthroline footprinting, quantitative footprinting, in vitro transcription assays in extract, gel mobility shift assays, western blot analysis, molecular cloning, hybridization analysis.
This expert is highly experienced in areas including DNA and RNA sequencing, radioisotope usage and small animal dissection, influenza virus receptors, slow viruses, Scrapie, Mad Cow and Kuru, UV-light and apoptotic signal transduction, nervous system disorders, Alzheimer's disease, mouse hippocampus, RhoB mRNA and UV light, phosphorylation of xenopus transcription factor IIIA, extracellular regulated kinase controls, upregulation of nucleolin mRNA and protein, inhibition of RNA polymerase III transcription, amyloid precursor protein (APP) production, neurodegenerative disorders, RNA binding protein HuR, ribosomes and the transcription of RNA Polymerase III genes.
PROFESSIONAL EXPERIENCEConsultant, Kevin Kennedy Associates Inc. Providing chemistry consulting, biochemistry consulting, biomedical research, and related expertise to a wide variety of clients. Chemist, Biochemist, a major research university Researched the Identification of Novel FMRP mRNA Targets in Neurodegenerative Disorders. Techniques Employed: mouse brain dissection, synaptosome preparation, neuronal cell culture, mouse breeding and flow cytometry.
Researched the Post-Transcriptional Regulation of RhoB mRNA by the RNA Binding Protein HuR. Techniques Employed: cell culture and transfection, cell fractionation, northern and western blotting, immunoprecipitation, recombinant DNA cloning, RT-PCR, fluorescent microscopy and sequence alignment analysis.
Researched the Isolation of Novel Early Response Genes from the Central Nervous System in a Mouse Epilepsy Model. Techniques Employed: bacterial protein expression, mRNA isolation, in vitro AUrich RNA selection by affinity chromatography, cDNA synthesis, cDNA library screening, BLAST homology searches.
Additional Training
Mentor to graduate and undergraduate students, training in mouse handling and preparation of R01 grant applications. Postdoctoral Research Fellow, Department of Pathology & Laboratory Medicine and Institute on Aging, University of Wisconsin-Madison Researched The Molecular Biology of Alzheimer’s Disease. Techniques Employed: particle mediated gene transfer, PBMC isolation, in vitro transcription and RNA purification, northern hybridization analysis, SDS-PAGE and ECL western blot analysis, ELISA, PCR, RNA gel mobility shift assays, cell culture, protein purification and tat-mediated protein transfection, protein kinase assays, sequence analysis Postdoctoral Research Associate, Department of Chemistry and Biochemistry, University of Notre Dame Researched Continuation of Dissertation Projects described below Graduate Student, Biophysics, and Molecular Biology Training Program, Department of Chemistry and Biochemistry and Biochemistry, University of Notre Dame Self-Directed Dissertation Project: The Phosphorylation of Xenopus Transcription Factor IIIA by an Oocyte Casein Kinase. Techniques Employed: in vitro phosphorylation, protein digestion and peptide isolation, amino acid hydrolysis, identification of phosphoamino acids by thin layer chromatography, oocyte isolation and in vivo radiolabeling of proteins, affinity purification of antibodies, immunoprecipitation, expression and purification of recombinant protein in E.coli, SDS-PAGE, autoradiography and densitometry
Primary Dissertation Project: The Effect of Ribosomes on the Transcription of RNA Polymerase III Genes. Techniques Employed: protein and nucleic acid purification, isolation of eukaryotic ribosomes, electrophoresis, germinal vesicle isolation, preparation of whole cell and nuclear transcription extracts, DNase I and copper phenanthroline footprinting, quantitative footprinting, in vitro transcription assays in extract, gel mobility shift assays, western blot analysis, molecular cloning, hybridization analysis, DNA and RNA sequencing
Additional Training
Certification in radioisotope usage and small animal dissection. PUBLICATIONSNumerous publications on topics related to mouse hippocampus, RhoB mRNA and UV light, phosphorylation of xenopus transcription factor IIIA, extracellular-regulated kinase controls, upregulation of nucleolin mRNA and protein, inhibition of RNA polymerase III transcription, amyloid precursor protein (APP) production, and more.
Numerous presentations on topics related to UV-light and apoptotic signal transduction, nervous system disorders, Alzheimer's disease, and more. Case Studies
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